Androstenedione Saliva Kit


$233.00

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SKU: SLV-4780 Categories: ,

Description

INTRODUCTION
Competitive immunoenzymatic colorimetric method for the quantitative measurement of Androstenedione concentration in saliva.
Androstenedione Saliva kit is intended for laboratory use only.

PRINCIPLE

  • Androstenedione (antigen) in the sample competes with the antigenic Androstenedione conjugated with horseradish peroxidase (HRP) for binding onto the limited number of antibodies anti- androstenedione coated on the microplate (solid phase).
  • After incubation, the bound/free separation is performed by a simple solid-phase washing.
  • Then, the enzyme HRP in the bound-fraction reacts with the Substrate (H2O2) and the TMB Substrate and develops a blue color that changes into yellow when the Stop Solution (H2SO4) is added.
  • The colour intensity is inversely proportional to the Androstenedione concentration of in the sample.
  • Androstenedione concentration in the sample is calculated through a standard curve.

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