Description
The Chikungunya IgM- µ-capture ELISA is intended for the qualitative determination of IgM class antibodies to Chikungunya virus in human serum and plasma (citrate).
The qualitative immunoenzymatic determination of IgM-class antibodies to Chikungunya is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are precoated with anti human IgM to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample and control material Chikungunya antigen solution is added. After a further washing step a mixture of biotinylated Chikungunya antibody and Streptavidin conjugate is added. After washing the captured Chikungunya-specific immuncomplex is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Chikungunya-specific IgM antibodies in the patient specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.
