Description
An enzyme immunoassay for the quantitative measurement of connective tissue growth factor (CTGF) in serum.
CTGF, a member of the CCN protein family, is relevant in many physiological and pathological processes (1). Both, clinical and experimental studies have demonstrated that CTGF is overexpressed in fibrotic human liver and in experimental animal models of liver fibrogenesis (2). CTGF is detectable in various human fluids (serum, plasma, urine) and can
potentially provide information about fibrotic remodelling processes (3).
The DRG CTGF ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a polyclonal
[goat] antibody directed towards a unique antigenic site of the CTGF molecule.Together with assay buffer an aliquot of patient sample containing endogenous CTGF is incubated in the coated well. After incubation the unbound material is washed off. A second incubation with antiserum and a washing step follows. Enzyme conjugate, which is a goat anti rabbit antibody conjugated with horseradish peroxidase is added. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the
concentration of CTGF in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of CTGF in the patient sample.
