Immunoenzymatic colorimetric method for quantitative measurement of IgA in saliva.
Salivary IgA ELISA is intended for laboratory use only.
- Salivary IgA ELISA is based on the simultaneous binding of human IgA to two antibodies, one monoclonal immobilized on microwell plates and the other, polyclonal conjugated with horseradish peroxidase (HRP). After incubation the bound/free separation is performed by a simple solid-phase washing.
- Then the enzyme in the bound-fraction reacts with the Substrate (H2O2) and the TMB Substrate and develops a blue color that changes into yellow when the Stop Solution (H2SO4) is added.
- The color intensity is proportional to the IgA concentration in the sample.
- The IgA concentration in the sample is calculated through a standard curve.