The DRG Influenza Virus A IgG-ELISA is for the quantitative determination of IgG class antibodies to Influenza virus A in human serum or plasma (citrate).
The immunoenzymatic determination of IgG-class antibodies to Influenza Virus A is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique.
Microtiter strip wells are precoated with Influenza Virus A antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added. This conjugate binds to the captured Influenza Virus A specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Influenza Virus A specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450nm is read using an ELISA microwell plate reader.