Immunoenzymatic colorimetric method for quantitative determination of hNSE concentration in human serum.
NSE ELISA kit is intended for laboratory use only.
Neuron Specific Enolase (2-phospho-D-glycerate hydrolase) is an isoenzyme that belongs to the enolase family (homo- and heterodimer constituted of _, _ and _ subunit) that is distingued from these by the presence of the specific __ heterodimer. The clinical usefullness of hNSE like tumor marker is compared to non small cell lunge cancer (NSCLC), to neuroblastoma, to medullary carcinoma of the thyroid, pancreatic islet cell tumor and to non neoplastic condition of neuronal disease and cerebral trauma. The NSE ELISA test cannot be used as a screening test for neuroendocrine tumors, but may be used to follow levels in established diagnosis.
The NSE ELISA test is based on simultaneous binding of human Neuron Specific Enolase by two monoclonal antibodies, one immobilized on microwell plates and the other conjugates with horseradish peroxidase (HPR). After incubation the bound/free separation is performed by a simple solid-phase washing, then the TMB-Substrate solution (TMB) is added. After an appropriate time has elapsed for maximum colour development, the enzyme reaction is stopped and the absorbancies are determinated. The colour intensity is proportional to the hNSE concentration in the sample. hNSE concentration in the sample is calculated based on a Calibration curve.