An enzyme immunoassay for the quantitative measurement of human placenta growth factor (PLGF) in serum.

Agiogenesis and vascular transformation are important processes in the normal development of the placenta. Abnormal angiogenesis and vascular transformation are considered to be one of the main reasons for preeclamptic pregnancies and intrauterine growth retardation. Placental growth factor PLGF, a member of the VEGF family, is produced mainly by the placenta and is a potent angiogenic factor. The corresponding receptor, the soluble fms-like tyrosine kinase-1 is considered to have angiogenic properties.

The DRG PLGF ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal antibody directed towards a unique antigenic site of the PLGF molecule. An aliquot of patient sample containing endogenous PLGF is incubated in the coated well. After a
washing step a biotin-linked polyclonal antibody specific for PLGF is added to the wells. Following a wash to remove any unbound antibody a streptavidin HRP enzyme complex is added to the wells. After incubation the unbound enzyme complex is washed off. The amount of bound peroxidase is proportional to the concentration of PLGF in the sample.
Having added the substrate solution, the intensity of colour developed is proportional to the concentration of PLGF in the patient sample