Description
The DRG Retinol Binding Protein (RBP) kit is designed to quantitatively measure RBP present in serum and plasma samples. Please read the complete kit insert before performing this assay.
The DRG Retinol Binding Protein (RBP) kit is designed to quantitatively measure RBP present in serum and plasma samples. Please read the complete kit insert before performing this assay. A RBP standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A RBP-peroxidase conjugate is added to the standards and samples in the wells. The binding
reaction is initiated by the addition of the RBP polyclonal antibody to each well. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound RBP-peroxidase conjugate. After a short incuba_tion, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the RBP in the sam_ple is calculated, after making a suitable correction for the dilution of the sample, using software available with most plate readers.
