Description
An enzyme immunoassay for the detection of IgG antibodies to rubella virus in serum.
Purified Rubella antigen is coated on the surface of microwells.Diluted serum is added to the wells, and the Rubella IgG- specific antibody, if present, binds to the antigen during incubation.After washing the wells to remove unbound sample, antibody to human IgG conjugated with horseradish peroxidase (HRP) is added and incubated at 37°C for
30 minutes. Unbound conjugate is removed by a subsequent washing step. A solution of TMB Reagent is then added to the microwells. The enzyme conjugate catalytic
reaction is stopped at a specific time.The intensity of the color generated is proportional to the amount of IgG-specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrators and controls.
