Description
The Sperm Antibody ELISA from DRG is a reliable and quantitative test for the determination antibodies directed against human spermatozoa. This test is intended for the use with seminal plasma.
Please note: the terms Òanti-spermatozoa antibodiesÓ, Òanti-sperm antibodiesÓ and Òsperm antibodiesÓ are equivalent. In these instructions the rather unwieldy but correct term Òanti-spermatozoa antibodiesÓ is used.
The Sperm Antibody ELISA is a part in clinical practice to diagnose immunologically caused infertility in men.
The DRG Sperm Antibody ELISA (Enzyme Linked ImmunoSorbent Assay) is a solid-phase sandwich enzyme-immunoassay for the quantitative determination of anti-spermatozoa antibodies in human seminal plasma. The ELISA-plate is coated with a mix of spermatozoa proteins which are recognized by anti-spermatozoa antibodies. The samples and standards are pipetted into the wells and then incubated. During this incubation anti-spermatozoa antibodies bind to the spermatozoa proteins and are thus immobilised on the plate. After washing the enzyme conjugate, consisting of anti-human globulin antibodies covalently coupled to horseradish peroxidase, is added. After removal of the unbound conjugate by washing the horseradish peroxidase oxidizes the then added substrate TMB (3,3Õ,5,5Õ-tetramethylbenzidine) yielding a colour reaction which is stopped with 0.25 M sulphuric acid (H2SO4). The extinction is measured at a wavelength of 450 nm with a microplate reader. The use of a reference measurement with a wavelength ³550 nm is recommended.
