An enzyme immunoassay for the quantitative measurement of TGF__1 in serum and cell culture supernatant.

Transforming Growth Factor _1 (TGF-_1) is a 25 kDa Homodimer composed of two 12.5 kDa subunits joined by disulfide bonds. TGF-_1 is a multipotent Cytokine with cell- and dose-dependent activities. This molecule is produced by a number of cells and tissue types, e.g. thrombocytes, bone tissue, placenta and kidneys. This potent Cytokine modulates embryonic development, bone formation, mammary development, wound healing, hematopoiesis, cell cycle progression and the production of the extracellular matrix. With respect to the immune system, TGF-_1 inhibits T and B cell proliferation and acts as an anti-inflammatory molecule both in vitro and in vivo. TGF-_1 inhibits macrophage maturation and activation. This molecule also inhibits the activity of natural killer cells and lymphokine activated killer cells and blocks cytokine production.

The DRG TGF__1 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. Prior to testing the standards and samples are diluted in assay buffer, acidified with HCl and then neutralized with Neutralization Buffer. Thereafter, the neutralized standards and samples are added to the antibody coated (polyclonal) microtiter wells. After incubation unbound sample material is removed by washing. In a second step monoclonal mouse anti TGF-_1 antibody, a biotinylated anti mouse IgG antibody and the Streptavidin-HRP Enzyme complex are incubated successively, forming an immuno enzyme sandwich complex. After incubation the unbound conjugate is washed off. Having added the substrate solution, the intensity of color developed is proportional to the concentration of TGF _1 in the sample.