An enzyme immunoassay for the quantitative measurement of transforming growth factor _2 (TGF__2) in serum, plasma, and cell culture supernatant.

Transforming growth factor-_ (TGF__) is a multipotent Cytokine with cell- and dose-dependent activities. This molecule is produced by a number of cells and tissue types, e.g.
thrombocytes, bone tissue, placenta and kidneys. TGF-_2 is not produced by blood platelets, contrary to TGF__1. This potent Cytokine modulates embryonic development, bone formation, mammary development, wound healing, hematopoiesis, cell cycle progression and the production of the extracellcular matrix. TGF__2Ð null mice were shown to exhibit perinatal mortality and a wide range of developmental defects for a single gene description which include cardiac, lung, craniofacial, limb, spinal column, eye, inner ear and urogenitial defects. TGF__2 has been shown to be a potent growth inhibit factor in the regulation of postnatal cerebellar neurons and neuroblast proliferation. TGF__2 has been
detected in tear fluid. TGF__2 levels are elevated in the vitreous of patients with proliferative diabetic retinopathy. Elevated plasma levels of TGF__2 have been described in patients with disseminated malignant melanoma. TGF__2 concentrations are furthermore elevated in ParkinsonÕs disease in ventricular cerebrospinal fluid.

The DRG TGF__2 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. Prior to testing the standards and patient samples are diluted in assay buffer, acidified with HCl and then neutralized with NaOH. Afterwards, the neutralized standards and samples are added to the antibody coated (polyclonal) microtiter wells. After the first incubation the unbound sample material is removed by washing. Then a biotinilated mouse anti TGF-_2 antibody and the Streptavidin-HRP Enzyme complex are incubated in succession. An immuno enzyme sandwich complex is formed. After incubation the unbound conjugate is washed off. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of TGF__2 in the patient sample.