Description
An enzyme immunoassay for the qualitative and semiquantitative determination of IgG-class antibodies to ureaplasma urealyticum in serum and plasma.Ureaplasma urealyticum is a bacterium belonging to the family Mycoplasmataceae. This microorganism is part of the normal genital flora of both men and women. It is found in about 70% of sexually active humans. It can cause disease, including non-specific urethritis (NSU), infertility, chorioamnioitis, stillbirth, premature birth, and, in the perinatal period, pneumonia or meningitis.
The DRG Ureaplasma urealyticum IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Microtiter wells as a solid phase are coated with Ureaplasma urealyticum UreD protein. Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Ureaplasma urealyticum-specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti_IgG conjugate binds specifically to IgG antibodies resulting in the formation of
enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.The intensity of this color is directly proportional to the amount of Ureaplasma urealyticum-specific IgG antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.
