Description
VitaminB1 Test is a microtiter plate test kit based on a microbiological assay which measures the VitaminB1 content in whole blood. The test kit contains all required reagents, e.g. standard, medium and microtiter plate coated with a specific microorganism, sufficient for 96 determinations including standard curves. An ELISA reader is required for evaluation of the VitaminB1 content.
The bioactive form of vitamin B1 is thiamine pyrophosphate. It plays an important role as a co-enzyme in carbohydrate and amino acid metabolism. Thiamine pyrophosphate is a vital co-factor for enzymes involved in several key metabolic processes in the nervous system, the heart, the blood cells, and the muscle. VitaminB1 assists in the conversion of carbohydrates into energy, necessary for healthy brain and nerve cells and heart function.
The blood samples are enzymatically pre-treated and then transferred in the wells of a microtiter plate coated with Lactobacillus fermentum. The addition of vitamin B1 in either Standards, controlor samples gives a vitamin B1-dependent growth response until vitamin B1 is consumed.
After incubation at 37 °C for 48 hr, the growth of Lactobacillus fermentum is measured turbidimetrically at 610 – 630 nm (alternatively at 540 – 550 nm) in an ELISA-reader and a standard curve is generated from the dilution series. The amount of vitamin B1 is directly proportional to the turbidity.
