The Cotinine ELISA is intended for the screening for the presence of Cotinine in human serum.For professional forensic use only.
A positive test result should be confirmed by a second test method such as GCMS (gas chromatography-mass spectrometry) or LCMS (liquid chromatography-mass spectrometry).
The Cotinine ELISA is a competitive enzyme immunoassay for the detection of Cotinine in human serum, at a cut-off concentration of 25 ng/mL. The wells of the microtitre strips are coated with anti-Cotinine antibody. During the first incubation, the horseradish peroxidase (HRP) labelled Cotinine competes with the free Cotinine in the donors sample
for the anti-Cotinine antibody binding sites on the microtitre strips. The wells are washed to remove any excess enzyme material prior to the addition of the TMB substrate solution. Addition of the stop solution terminates the reaction and absorbances are read spectrophotometrically at 450nm.