The Fibrinogen test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Fibrinogen in biological samples of humans.
Soluble Fibrinogen (FIB) circulates in the blood and provides the material from which the insoluble fibrin clot is formed during blood coagulation. Fibrinogen is an acute phase reactant that may be a useful marker for infection and inflammation. This ELISA kit can be used to measure Fibrinogen in biological samples.
The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Fibrinogen present in samples reacts with the anti-Fibrinogen antibodies which
have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-FIB antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound FIB. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3Õ,5,5Õ-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of FIB in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of FIB in the test sample. The quantity of FIB in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.