Description
The Influenza Virus B IgM-ELISA is intended for the qualitative determination of IgM class antibodies to Influenza Virus B in human serum or plasma (citrate).
The qualitative immunoenzymatic determination of IgM-class antibodies to Influenza Virus B is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are precoated with Influenza Virus B antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material
horseradish peroxidase (HRP) labelled anti-human IgM conjugate is added. This conjugate binds to the captured Influenza Virus B specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Influenza Virus B specific IgM antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader
