Description
The Rotavirus Ag ELISA is an in-vitro-diagnostic device for direct detection of Rotavirus in faecal samples.
TheRotavirus Ag ELISA is a one-step enzyme immunoassay on the basis of polyclonalantibodies to the group specific VP-6 antigen, the major protein of group ARotaviruses.Dilutedstool specimens and horseradish peroxidase (HRP) labelled polyclonalanti-Rotavirus-antibodies are dispensed simultaneously into the wells of amicrotitration plate coated with polyclonal anti-Rotavirus antibodies.Afteran incubation time of 60 min at room temperature unbound components are removedby a washing step.HRP converts the subsequently added colorless substrate solutionof 3,3Õ,5,5Õ-Tetramethylbenzidine (TMB) within a 10 min reaction time into ablue product. The enzyme reaction is terminated by sulphuric acid dispensedinto the wells turning the solution from blue to yellow.The optical density (OD) of the solution read at 450/620 nm isdirectly proportional to the specifically bound amount of Rotavirus.Considering the cut-off value results are interpreted as positiveor negative.
