Description
TPS ELISA is intended for determination of cytokeratin 18 in serum.
TPS ELISA is a one step enzyme linked sandwich immunoassay. Standards, controls and samples react during incubation simultaneously with a solid phase monoclonal catcher antibody and the HRP-conjugated detector antibody (M3). After washing, the TMB substrate is added and after an incubation time the reaction is stopped and the absorbance at 450 nm is measured. The developed colour is directly proportional to the concentration of the analyte.
